Improved Process for Making mRNA-Loaded Lipid Nanoparticles

摘要

The present invention provides improved methods for formulating lipid nanoparticles and encapsulating mRNA. In some embodiments, the present invention provides a method of encapsulating messenger RNA (mRNA) with preformed lipid nanoparticles, the method comprising mixing a solution of the preformed lipid nanoparticles with a low concentration of mRNA.