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Identification and isolation of human corneal endothelial cells (HCEC)

机译:人角膜内皮细胞(HCEC)的鉴定和分离

摘要

Problem to be solved: to provide a method for identification, separation and / or enrichment of human corneal endothelial cells (HCEC).The method isCell populations containing human corneal cellsCells other than HCEC within the cell populationA positive screening process that is in contact with a positive affinity reagent that selectively binds to HCEC in comparison with corneal stromal cells, etcAnd a cell population containing HCECIt includes a negative screening process that is contacted with a negative affinity reagent that selectively binds to cells other than HCEC within the cell population compared to HCEC.No selection
机译:要解决的问题:提供用于鉴定,分离和/或富集人角膜内皮细胞(HCEC)的方法。含有除HCEC之外的人体角膜细胞的方法含有除HCEC之外的人类角膜型筛选方法,该筛选方法与与角膜基质细胞相比,含有Hcecit的细胞群具有相比之与HCEC的阳性亲和试剂相比,含有HCecit的细胞群包括阴性筛选过程与HCEC.NO选择相比,与阴性亲和试剂接触的负亲和试剂,其选择性地结合细胞群中的HCEC以外的细胞

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