The present disclosure includesAbout the method for culturing voldeella speciesThe method: culturing voldeella species in a liquid medium under aerobic conditions;And by using strong acids such as nitric acidOr, the first and second acids may be used to maintain the pH of the liquid mediumHere is the first acidNitric acidIt is an inorganic acid inherently dissociated completely in water such as hydrochloric acid or sulfuric acidAnd here the second acid isIt is an inorganic acid having an acid dissociation constant (PKA) greater than 1, such as phosphoric acid.Methods are also provided to increase the yield of bordeera hairy agglutination 2 and fimbrial agglutination 3 (fim2 / 3) in supernatant fractions from bordeera species cultures.
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