HIERARCHICAL SPHEROID BLOOD BRAIN BARRIER MODEL USING HUMAN IMMORTALIZED CELL AND METHOD FOR PRODUCING SAME

摘要

Provided is a method for producing a multicellular, three-dimensional blood brain barrier model, comprising: (i) a first seeding step for seeding a human conditionally immortalized astrocyte and a human conditionally immortalized pericyte on a medium; (ii) a first coculturing step for coculturing the seeded human conditionally immortalized astrocyte and human conditionally immortalized pericyte to obtain a coculture containing two types of cells; (iii) a second seeding step for seeding a human conditionally immortalized brain microvascular endothelial cell on a medium that contains the coculture containing the two types of cells; and (iv) a second coculturing step for coculturing the coculture containing the two types of cells and the human conditionally immortalized brain microvascular endothelial cell to obtain a multicellular, three-dimensional blood brain barrier model.