首页>
外国专利>
DROPLET-BASED SINGLE CELL GENOMIC DNA SEQUENCING
DROPLET-BASED SINGLE CELL GENOMIC DNA SEQUENCING
展开▼
机译:基于液滴的单细胞基因组DNA测序
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present disclosure relates to compositions and methods for performing droplet-based high throughput sequencing upon genomic DNA of single cells (e.g., individual sperm). The instant disclosure provides a droplet that includes: i) a mammalian cell nucleus; and ii) a microbead presenting attached oligonucleotides, where the attached oligonucleotides include a nucleic acid sequence capable of hybridization and capture of genomic DNA and a microbead identification sequence that is common to all oligonucleotides attached to the microbead, where the mammalian cell nucleus is accessible to the microbead-attached oligonucleotides to an extent sufficient to allow for genomic DNA capture and amplification of genomic DNA to occur within the droplet.
展开▼