THERMOSTABLE PETASE ENZYME

摘要

The invention discloses a cyclic PETase enzyme, which is enzymatically active and has enhanced thermostability. In a preferred embodiment, a polyhistidine-G4S flexible linker connects SpyCatcher to PETase while a protease cutting site/linker connects PETase to SpyTag, and wherein a lysine (K) residue on the SpyCatcher is able to form an isopeptide bond with an aspartate (D) residue on the Spytag.