GELATIN-BASED MICRONEEDLE PATCH FOR MINIMALLY-INVASIVE EXTRACTION OF BODILY FLUIDS

摘要

A gelatin-based (e.g., gelatin methacryloyl (GelMA)) patch is disclosed with an array of microneedles (MNs) for minimally invasive sampling of bodily fluids such as interstitial fluid (ISF). The properties of the patch can be tuned by altering the concentration of the GelMA prepolymer and the crosslinking time. The GelMA-based MN patch demonstrated efficient extraction of ISF. Furthermore, in experimental testing, the patch efficiently and quantitatively detects glucose and vancomycin in ISF in an in vivo study. This minimally invasive approach of extracting ISF with a GelMA microneedle enables to detection and analysis of target molecules from patients. The target molecules captured in the patch may be released and analyzed to detect the presence of and/or concentration of target molecules. In other embodiments, the patch itself may be analyzed directly to detect the presence of and/or concentration of target molecules.