Polypeptides with phase transition, triblock polypeptides of the polypeptide-calmodulin-polypeptide with multi-stimuli responsiveness, hydrogel of the triblock polypeptides, and its uses

摘要

Disclosed is a polypeptide having a phase transition behavior, wherein the polypeptide consists of a Val-Pro-Gly-Xaa-Gly (SEQ ID NO: 1) pentapeptide repeat or a Val-Pro-Ala-Xaa-Gly (SEQ ID NO: 2) pentapeptide repeat, and the polypeptide includes a [Val-Pro-Gly-Xaa-Gly (SEQ ID NO: 1)]n or a [Val-Pro-Ala-Xaa-Gly (SEQ ID NO: 1)]n pentapeptide repeat. In addition, the present invention provides a multi-stimuli polypeptide composed of polypeptide-calmodulin-polypeptide having a phase transition behavior and a hydrogel prepared using the same. A dynamic protein hydrogel according to the present invention may be used as a drug carrier, as a scaffold for tissue engineering or as a kit for tissue or organ regeneration.