Enrichment and extraction method of nucleic acid for detecting foodborne pathogenic bacteria in food

摘要

The present invention comprises the steps of (a) adding an enrichment medium to a sample and culturing to obtain an enrichment solution; (b) adding a lysis buffer to the pellet obtained by first centrifuging the enrichment solution and incubating to obtain a lysis solution; (c) removing the flow-through by putting the lysate in a column and performing a second centrifugation; (d) adding a washing buffer to the lysate from which the flow-through is removed after the second centrifugation and performing a third centrifugation to remove the flow-through; and (e) adding an elution buffer to the lysate from which flow-through has been removed after the third centrifugation and performing a fourth centrifugation to extract the nucleic acid. It relates to a method for extracting nucleic acids from food poisoning bacteria. . The nucleic acid extraction method of the present invention is a nucleic acid extraction method for detecting food poisoning bacteria commonly found in meat or processed meat products, particularly Listeria monocytogenes . Extraction time is shortened, and nucleic acid extraction can be performed with high purity and yield at room temperature without the need to adjust the optimum temperature of the enzyme because no enzyme is used.