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ULTRASENSITIVE METHOD FOR MULTIPLEXED DETECTION OF BIOMARKERS
ULTRASENSITIVE METHOD FOR MULTIPLEXED DETECTION OF BIOMARKERS
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机译:用于多路复用检测生物标志物的超敏感方法
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摘要
The present invention relates to an ultrasensitive method for multiplexed detection of biomarkers. More specifically, the present invention provides a method for multiplexed detection of biomarkers, including a) attaching one or more types of biomarkers present in a sample taken from a subject to the surface of a substrate, b) attaching docking strands to the biomarkers and allowing detection antibodies to specifically bind to the biomarkers, and binding the detection antibodies to the corresponding biomarkers, c) binding imager strands labeled with fluorescent molecules or combinations of donor and acceptor strands to the docking strands to generate a fluorescence signal, and d) detecting the fluorescence signal. Steps c) and d) are repeated as many times as the number of the biomarker types by removing the used imager strands or the used donor and acceptor strands and introducing new imager strands or different combinations of donor and acceptor strands to the docking strands.
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