A three-dimensional gift box with acousto-optic effect includes a box body and a box lid; a first partition board and a second partition board which are provided in the box body, the first partition board is fixedly fitted to the inner edge of the box body, the second partition board is detachably fitted to the inner edge of the box bod; a circuit layer, which is formed in the space between the first partition board and the bottom plate of the box body; a storage layer, which is formed in the space between the first partition board and the second partition board; a foldable pop-up card, which is arranged between the second partition board and the inner side of the box lid; an electronic assembly module for producing the acousto-optic effect, which is arranged in the circuit layer.
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