METHOD FOR CONSTRUCTING SEQUENCING TEMPLATE BASED ON IMAGE, AND BASE RECOGNITION METHOD AND DEVICE

摘要

A method for constructing a sequencing template based on an image, a device, and a system. The image comprises first, second, third and fourth images of one same field of view corresponding to base base extensions of A/U, T, G, and C respectively; the first, second, third and fourth images respectively comprise images M1 and M2, images N1 and N2, images P1 and P2, and images Q1 and Q2; the method for constructing the sequencing template comprises: combining any two of the images M1, M2, N1, N2, P1, P2, Q1, and Q2 to perform bright spot matching, and enabling the images M1, N1, N2, P1, P2, Q1, and Q2 to participate in the combination for at least one time to obtain a plurality of combined images comprising first coincident bright spots (S10), two or more bright spots whose distances are less than that of a first predetermined pixel on the combined images being one first coincident bright spot; and merging the first coincident bright spots on the plurality of combined images to obtain a bright spot set corresponding to the sequencing template (S20). The method can effectively obtain the bright spot set corresponding to a nucleic acid template.