(A) forming a first complex comprising:an analyte;a first trapping body which specifically binds to the analyte;a second trapping body which specifically binds to a site of the analyte, the site being different from a site to which the first trapping body specifically binds; anda third trapping body which specifically binds to the second trapping body;(B) separating a part comprising the analyte and the first trapping body from the third trapping body;(C) allowing a fourth trapping body to trap the part to form a second complex; and(D) detecting the analyte of the second complex,wherein the second trapping body comprises a binding substance which binds to the analyte, a support, and a linker which links the binding substance and the support with each other."/> Method for detecting analyte, detection reagent kit, and detection reagent
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Method for detecting analyte, detection reagent kit, and detection reagent

机译:检测分析物,检测试剂盒和检测试剂的方法

摘要

Disclosed is a method for detecting an analyte in a sample, including the steps of:(A) forming a first complex comprising:an analyte;a first trapping body which specifically binds to the analyte;a second trapping body which specifically binds to a site of the analyte, the site being different from a site to which the first trapping body specifically binds; anda third trapping body which specifically binds to the second trapping body;(B) separating a part comprising the analyte and the first trapping body from the third trapping body;(C) allowing a fourth trapping body to trap the part to form a second complex; and(D) detecting the analyte of the second complex,wherein the second trapping body comprises a binding substance which binds to the analyte, a support, and a linker which links the binding substance and the support with each other.
机译:公开了一种用于检测样品中分析物的方法,包括以下步骤: (a)形成第一个复杂,包括: 一个分析物; 特异性绑定到分析物的第一捕获体; 第二捕获体,其特异性地结合分析物的部位,该网站与第一捕获体特异性结合的网站不同;和 三个捕获体,它具体绑定到第二捕获体; (b)将包含分析物和第一捕获体的部分与第三捕获体分开; (c)允许第四捕获体捕获部分以形成第二个复合物; (d)检测第二个复杂的分析物, 其中第二捕获体包括结合物质,其结合与分析物,支撑件和连接粘合物质和彼此的载体的接头。

著录项

  • 公开/公告号US11047852B2

    专利类型

  • 公开/公告日2021-06-29

    原文格式PDF

  • 申请/专利权人 SYSMEX CORPORATION;

    申请/专利号US201615216988

  • 发明设计人 TOSHIHIRO WATANABE;SEIICHI HASHIDA;

    申请日2016-07-22

  • 分类号G01N33/543;G01N33/544;

  • 国家 US

  • 入库时间 2022-08-24 19:39:02

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