Provided is a method of constructing a DNA library for single cell genome sequencing, comprising, fragmenting DNAs in cell nuclei to obtain DNA fragmented cell nuclei; carrying out multiple rounds of marking on the fragmented DNAs in a plurality of cell nuclei by means of different sequence tags, so that the fragmented DNAs in each cell nucleus are connected with tag codes consisting of a plurality of sequence tags, and the tag codes connected with the fragmented DNAs of each cell nucleus are different; and amplifying the fragmented DNAs connected with the tag codes to obtain the DNA library for single cell genome sequencing.
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