Quantification of nucleosome modifications using chemically defined recombinant nucleosomes

摘要

The present invention is a variant, modified by covalent bond (histone protein or wound DNA), from a biological sample It relates to the use of recombinant / semi synthetic nucleosomes that possess histone and / or DNA modifications as reference reference materials for quantification of variant nucleosomes (collectively "modified nucleosomes" or "nucleosome modifications").Further, the present invention relates to a method of using an assay to accurately quantify single or combined nucleosome modifications as biomarkers of disease.