Problem to be solved: to provide a multilayer culture vessel observation system, a carriage apparatus, and a multilayer culture vessel observation apparatus capable of easily observing an object to be observed in a multi-layer culture vessel.Solution: a carburetor 20 capable of moving with a multi-layer culture vessel 30 having a plurality of trays mounted thereonA viewing device 10 capable of observing an object to be observed on each tray of a multi-layer culture vesselA multilayer culture vessel observation system havingCarriage 20 isThe multi-layer culture vessel 30 comprises a frame having a side exposed portion exposing the upper end to the lower end of two opposing sides of the vesselThe observation apparatusA storage part 14 for accommodating the carriage device 20 in a state in which the multi-layer culture vessel 30 is mounted isImage pickup device 11 having an optical system and outputting an image formed by the optical systemEquipped withWhen the carriage device 20 is provided with the multi-layer culture container 30 in the accommodation partSide exposedA multilayer culture vessel observation system disposed on the optical axis of the imaging device 11.
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