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Industrial keratinase via genetic engineering and use thereof
Industrial keratinase via genetic engineering and use thereof
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机译:通过基因工程和使用的工业角蛋白酶
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摘要
The invention relates to the technical field of industrial biotechnologies, and discloses a keratinase mutant with improved thermal stability and use thereof. The asparagine at position 181, the tyrosine at position 217, and the serine at position 236 in the keratinase derived from Brevibacillus parabrevis (CGMCC No. 10798) are engineered by site-direction mutation, and combined at random to obtain an enzyme with combined mutations. The invention realizes the remarkable improvement of the thermal stability of keratinase, and has good theoretical value and application prospect.
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