Human mesenchymal stem cell capable of retarding cellular senescence and resisting malignant transformation, preparation method and use thereof

摘要

Provides a method of preparing human mesenchymal stem cells capable of retarding cellular senescence and resisting malignant transformation.The preparation method isCodon, encoding the 253 amino acid residues of FoxO3 proteinMutations from codon encoding serine to codon encoding alanineCodon, encoding the 316th amino acid residue of FoxO3 proteinTwo mutations of mutations from codon encoding serine to codon encoding alanineIncludes steps for the genome of pluripotent stem cells.The recombinant mesenchymal stem cells prepared in the above method are further provided in the cell transplantation treatment.No selection