Alpha hemolysin variants and their uses

摘要

The variant of alpha hemolysin having at least one mutation, such as mutation to positive charge, is described herein.In certain embodiments, the mutation was found to be 0k, a1k, A1R, D2N, S3K, d4k, d4n, k8r, n47k, e70k, s106k, e111n, 127-131g, d128k, k147n, v149k in the mature wild type alpha hemolytic amino acid sequence; Selected from e287r, m2988a or combinations thereof.A composition comprising a variant of alpha hemolysin, a nanopore construct containing an alpha hemolytic variant, and a method of sequencing nucleic acid incorporating it.Diagram