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CULTURING METHOD FOR GENUS RHIZOBIUM BACTERIA

机译:根瘤菌属培养方法

摘要

Provided herein is a culturing method for bacteria of the genus Rhizobium whereby a low viscosity can be maintained in a culture solution during the culturing of and at the end of culturing of said bacteria, and that enables easy and efficient post-procedures, including concentration and separation of viable bacteria, among others. This is achieved by growing Rhizobium bacteria by liquid culture using a medium containing a disaccharide (for example, maltose, trehalose, lactose, sucrose) accounting for at least 25 mass % of the carbon source in the medium while maintaining a pH of 5.0 to 7.0 in the culture solution throughout the course of the culture, namely from the beginning of the culturing to the end of the culturing of said bacteria.
机译:本文提供了培养的细菌的培养方法根序,在培养所述细菌的培养期间可以在培养溶液中维持低粘度,并且能够轻松效率后程序,包括浓度和可行性细菌的分离等。通过使用含有二糖(例如麦芽糖,海藻糖,乳糖,蔗糖)的液体培养基通过液体培养生长根瘤菌(Rhizobium)细菌来实现,占介质中的至少25质量%的碳源在培养过程中,在培养液中保持5.0至7.0的pH值,即从培养到所述细菌的培养结束时。

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