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REGISTRATION TECHNIQUES FOR MULTIPLEXED FLUORESCENCE IN-SITU HYBRIDIZATION IMAGES
REGISTRATION TECHNIQUES FOR MULTIPLEXED FLUORESCENCE IN-SITU HYBRIDIZATION IMAGES
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机译:多路复用荧光原位杂交图像的登记技术
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摘要
For acquisition of spatial transcriptomic information, one or more filters are applied to deconvolution is performed on each image of a plurality of images to generate a plurality of filtered images, and registration of the plurality of filtered images is performed to generate a plurality of registered and filtered images. For each pixel of at least two pixels of the plurality of registered and filtered images, the pixel is decoded by identifying a code word from a plurality of code words in a code book that provides a best match to data values in the plurality of registered and filtered images for the pixel. For each pixel of at least two pixels, a gene is associated with the code word is determined and an indication that the gene is expressed at the pixel is stored.
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