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Kit for producing cleared biological specimens and method for producing cleared biological specimens
Kit for producing cleared biological specimens and method for producing cleared biological specimens
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机译:用于生产清除生物标本的试剂盒及其制备清除生物标本的方法
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摘要
A method and a kit are provided to produce a biological clear specimen within a short time and with a wide variety of biological specimens. The method includes the following steps: (1) treating a material to be cleared with an aqueous fixing solution containing paraformaldehyde or the like, a nonionic surfactant, an alkali, and optionally a buffering agent; (2) treating the material from step (1) or a conventionally fixed material with an aqueous clearing accelerator solution containing a nonionic surfactant and an alkali; and (3) treating the material produced in step (1) or (2) with an aqueous preservation solution containing a nonionic surfactant and a polyhydric alcohol. In each of the steps, the nonionic surfactant is present at a concentration of 1% or more. The kit includes solutions used in each of the steps of the method.
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