The present invention provides a Diels-Alderase and use thereof, and belongs to the field of gene engineering technology. The Diels-Alderase is MaDA, and its amino acid sequence and gene sequence are represented by SEQ ID Nos. 1 and 2, respectively. The present invention also provides MaDA-1 and MaDA-2, both of which are homologous proteins of MaDA, and their amino acid sequences are represented by SEQ ID Nos. 10 and 12, respectively. The present invention has discovered that MaDA and its homologous proteins from Morus alba can stereospecifically synthesize natural products of endo configuration, and prepare D-A type natural products and their analogs in vitro using chalcones and dehydroprenyl-containing compounds as substrates, which helps to develop and utilize the medicinal value of such natural products, and also provides a possibility to synthesize other six-membered ring-containing important chemical precursors or natural products.
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机译:本发明提供了一种Diels- alderase及其用途,属于基因工程技术领域。 Diels- alderase是MARA,其氨基酸序列和基因序列分别由SEQ ID NO.1和2表示。本发明还提供MADA-1和MADA-2,两者都是MARA的同源蛋白,它们的氨基酸序列分别由SEQ ID NO和12表示。本发明发现来自 Morus Alba的Mada及其同源蛋白质可以立体地合成内诺构型的天然产物,并使用含Chalco.cn和含脱氢细胞的化合物作为基材的体外制备Da型天然产物及其类似物,这有助于开发和利用这种天然产物的药物价值,并且还提供了合成其他含六元环的重要化学前体或天然产物的可能性。
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