Crosslinked magnetic agarose bead having enhanced physicochemical stability and preparation method thereof

摘要

The crosslinked magnetic agarose beads provided in one aspect of the present invention have improved chemical stability, mechanical strength, and thermal stability compared to conventional magnetic agarose beads, so that they can be used under severe conditions such as high pressure, high temperature, and very high or low pH. And, there is no leakage of magnetic nanoparticles from the magnetic agarose beads, Since the manufacturing process is simple due to the emulsion droplet gel formation method, when applied to protein separation by an affinity tag, it can be used as an affinity chromatography medium that can improve the separation efficiency and yield of proteins.