A method is provided for the denitrification of a substance having nitrate (NO3—) molecules therein. The method includes collecting waste organic material having fats, oils and grease (“FOG”) therein, and separating the FOG from the collected waste organic material. The FOG is mixed with a saponific reagent thereby initiating a saponification reaction to hydrolyze the FOG to fatty acid salts. A resultant FOG mixture (“RFM”) is formed having stratified layers of one or more fatty acid mixtures (“FAM”) and a glycerol fraction derived mixture (“GFDM”). The GFDM is mixed with the substance wherein heterotrophic bacteria use oxygen from the nitrate (NO3—) molecules to breakdown the GFDM thereby producing nitrogen gas.
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