METHODS AND KITS USING INTERNAL STANDARDS TO CONTROL FOR COMPLEXITY OF NEXT GENERATION SEQUENCING (NGS) LIBRARIES

摘要

Described herein are kits and methods for quantifying the amount of at least one nucleic acid of interest in a sample. The kits include a mixture of synthetic spike-in internal standards (IS) reagents, where the spike-in IS reagents comprise a complexity calibration ladder (CCL) that includes synthetic internal standard competitors for at least one target gene.