Internal standard reagent, method for evaluating reliability of environmental DNA measurement value, and conversion method of environmental DNA measurement value

摘要

Problem to be solved: To evaluate the reliability of the measurement result of the environmental DNA, and to compare the measured value obtained in various environmental DNA measuring methods, and to measure the measured value in a certain measuring method It is to be able to convert it to a measurement value obtained when measured by another measuring method.SolutionOne aspect of the invention is an internal standard reagent for evaluating or altering environmental DNA measurements.This internal standard reagent contains DNA of an internal standard organism that is different from the measured organism and is not contained in the environment in which the organism is inhabited.Another aspect of the present invention is a method for evaluating the reliability of environmental DNA measurements using an internal standard reagent containing a predetermined amount of internal standard DNA.Still another aspect of the invention is a method of converting an environmental DNA measurement value obtained from a first measuring method using an internal standard reagent containing an internal standard DNA to an environmental DNA measurement value obtained by a second measuring method different from the first measurement method.No selection