Method and composition for increasing somatic cell transfer (SCNT) efficiency by removing histone H3 lysine trimethylation

摘要

The present invention provides methods and compositions for improving the efficiency of somatic cell transfer (SCNT), as well as the generation of nuclear import ESC (ntesc) and transgenic and / or transgenic non human animals.A method for increasing the efficiency of somatic cell transfer (SCNT);Donor mammalian cellsRecipient cell oocyteOr mammalian SCNT embryosDonor mammalian cellsRecipient cell oocyteOr, in a mammalian SCNT embryo, contact with an active substance that reduces H3K9 me3 methylation;It increases the efficiency of SCNTIncluding stepsThe method of increasing the efficiency of the SCNT.Diagram