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Systems and methods for immobilizing extracellular matrix material on organ on chip, multilayer microfluidics microdevices, and three-dimensional cell culture systems
Systems and methods for immobilizing extracellular matrix material on organ on chip, multilayer microfluidics microdevices, and three-dimensional cell culture systems
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机译:用于固定芯片,多层微流体微生物和三维细胞培养系统器官器官内细胞外基质材料的系统和方法
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摘要
The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.
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