ICE-FREE VITRIFICATION AND NANO-WARMING OF LARGE TISSUE SAMPLES

摘要

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues and large blood vessels, and includes, for example, exposing the cellular material to a high concentration cryoprotectant formulation containing at least 0.5 mg/mL Fe mNPs, subjecting the cellular material to a preservation protocol in which ice-induced damage to the cellular material does not occur, and obtaining a cryopreserved cellular material that has been nanowarmed; wherein metabolic activity of the nanowarmed tissue is fully recovered to control values within two days of being rewarmed.