Novel fungal autoinducible expression system

摘要

The present invention provides for a system comprising: (a) a first nucleic acid encoding an α-factor receptor operatively linked to a first promoter, (b) a second nucleic acid encoding a recombinase operatively linked to a promoter which is activated by an α-factor receptor bound to an α-factor, and (c) a third nucleic acid encoding a gene of interest (GOI) flanked by a pair of recombinase recognition sequences, recognized by the recombinase, operatively linked to a second promoter. The present invention provides for a genetically modified fungal cell comprising the system of the present invention.