METHOD FOR PRODUCING DOUBLE STRANDED POLYNUCLEOTIDES BASED ON OLIGONUCLEOTIDES WITH SELECTED AND DIFFERENT MELTING TEMPERATURES

摘要

A method for identifying a group of single-stranded oligonucleotides for self-assembly into a double-stranded polynucleotide, the group comprising a plurality of overlapping complementary oligonucleotides, wherein each overlap between complementary oligonucleotides is selected to have a melting temperature (Tm) that differs from the melting temperatures of all other overlapping complementary oligonucleotides in the group.