CONSTRUCTION AND SEQUENCING DATA ANALYSIS METHOD FOR CTDNA LIBRARY FOR SIMULTANEOUSLY DETECTING VARIOUS COMMON MUTATIONS IN LIVER CANCER

摘要

The invention discloses a method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer. The library construction method and sequencing data analysis process established by the present invention have the following advantages: 1. Simultaneous detection of multiple mutation forms in liver cancer without capturing; 2. Suitable for efficient capture of ultra-small target regions; 3. The library may support 10-20 tests; 4. Ligate the DNA barcode to the starting ctDNA molecule during the library construction process, and cooperate with the biological information analysis process to achieve high specific detection of low-frequency mutations in ctDNA; 5. The library may be used for PCR hot spots detection and sequencing by a capture method at the same time, the added DNA barcode may effectively filter out false positive mutations and achieve high-specificity sequencing based on duplex. The invention has important clinical significance for early screening, disease tracking, efficacy evaluation, prognosis prediction and the like of liver cancer.