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Gene mutation detection kit and gene mutation detection method

机译:基因突变检测试剂盒和基因突变检测方法

摘要

PROBLEM TO BE SOLVED: To provide a kit for detecting mutant genes and a method for detecting mutant genes that can easily and with high sensitivity detect the existence of mutations in a region in which mutations are concentrated.;SOLUTION: The kit for detecting mutant genes is provided with: a probe that, when a plurality of independent regions R1 and R2 that do not overlap in an exon of a target gene are the wild-type base sequence, hybridizes thereto; a label indicating that the probe has hybridized to each of R1 and R2; and a primer pair designed to include all of R1 and R2 in one amplicon obtained by PCR.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2018,JPO&INPIT
机译:要解决的问题:提供用于检测突变基因的试剂盒和用于检测可以容易且高灵敏度的突变基因的方法检测突变浓缩的区域中的突变存在。;溶液:用于检测突变基因的试剂盒提供:探针,当在靶基因的外显子中不重叠的多个独立区域R1和R2是野生型碱基序列时,与其杂交;表示探针与R1和R2中的每一个杂交的标签;设计用于在通过PCR获得的一个扩增子中包含所有R1和R2的引物对。所选择的绘图:图1;版权:(c)2018,JPO和INPIT

著录项

  • 公开/公告号JP6871598B2

    专利类型

  • 公开/公告日2021-05-12

    原文格式PDF

  • 申请/专利权人 国立大学法人広島大学;

    申请/专利号JP20170006625

  • 发明设计人 檜山 英三;

    申请日2017-01-18

  • 分类号C12N15/11;C12Q1/6827;C12Q1/6876;

  • 国家 JP

  • 入库时间 2022-08-24 18:38:26

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