MULTI-FLUID DENSITY GRADIENT COLUMNS

摘要

The present disclosure includes a method of forming and loading a multi-fluid density gradient column. The method can include forming a multi-fluid density gradient column and loading magnetizing microparticles into a first fluid layer or a second fluid layer of the multi-fluid density gradient column. Forming the multi-fluid density gradient column can include loading a first fluid having a first fluid density in a multi-fluid density gradient column to form a first fluid layer and loading a second fluid having a second fluid density greater than the first fluid density in the multi-fluid density gradient column to form a second fluid layer. The multi-fluid density gradient column can be fluidly coupled to a fluid processing device. The magnetizing microparticles can be surface-activated to bind with a biological component or can be bound to the biological component.