Lipid conjugated genome editing protein-based CRISPR complex and fabrication method thereof

摘要

The present invention relates to a CRISPR complex based on a gene-editing protein conjugated with a lipid, a method for preparing the same, and a use thereof, and more specifically, a ribonucleic acid protein complex including a CRISPR enzyme protein and sgRNA modified by directly conjugating a lipid, and preparation thereof. It relates to a method, and a gene editing application using the same. According to the present invention, the CRISPR complex is an efficient and safe non-viral gene editing means capable of solving problems such as conventional non-specific editing, gene mutation, biotoxicity, and low gene editing efficiency. It is expected to be useful in various fields such as.