Genomic editing vector for Eubacterium callanderi, Method for editing genome of Eubacterium callanderi using the same, and Transgenic Eubacterium callanderi strains using the same

摘要

An embodiment of the present invention provides a genome editing vector for Eubacterium calanderi for the CRISPR/Cas9 system. The genome editing vector for Eubacterium calanderi according to an embodiment of the present invention includes a DNA sequence encoding a guide RNA (gRNA) of a target gene for cutting; DNA sequence encoding the Cas9 protein; _{A P rbo} promoter operably linked to a DNA sequence encoding the Cas9 protein; Origin of replication derived from E. coli; Origin of replication for Eubacterium calanderi; And a marker for selecting a transformed strain. According to an embodiment of the present invention, a genome editing vector for Eubacterium calander, applicable to Eubacterium calanderi strain, which is an acetogen suitable for syngas bioconversion process, and a method for editing the genome of Eubacterium calanderi strain using the same, and This can be used to provide a transformed Eubacterium calander strain.