(Problem) The present invention provides an improved β-fructopranosidase capable of suppressing the rate at which by-products such as nystose are produced and efficiently producing ketose. (Solution means) With respect to the amino acid sequence of β-fructopranosidase comprising an amino acid sequence having at least 60% identity to the amino acid sequence of wild-type β-fructopranosidase represented by SEQ ID NO: 2, the above sequence number in alignment An amino acid sequence in which an amino acid mutation in which an amino acid mutation is substituted for histidine (H) corresponding to the 395th position from the N-terminus of the amino acid sequence of wild-type β-fructopranosidase represented by 2 with arginine (R) or lysine (K). It is an improved β-fructopranosidase formed.
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机译：（问题）本发明提供一种改进的β-果加调解酶，其能够抑制产生副产物如肾系膜的速率和有效地生产酮糖的速率。 （溶液方法）关于β-果加调解酶的氨基酸序列，其包含具有由SEQ ID NO：2表示的野生型β-果加调解酶的氨基酸序列具有至少60％同一性的氨基酸序列，上述序列号在对准中，其中氨基酸突变，其中氨基酸突变被氨基酸突变取代的组氨酸（H）对应于来自2的野生型β-果加权酶的氨基酸序列的N-末端的第395个位置用精氨酸（R）或赖氨酸（K）。它是形成的改进的β-果加权酶。
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