Compositions and methods for improving library enrichment

摘要

The present disclosure describes a hybridization buffer comprising a clustering agent, a method comprising using the hybridization buffer, and a kit comprising the hybridization buffer. The present disclosure also describes blocking agents for use in hybrid capture methods, methods of using such blocking agents, and kits comprising such blocking agents. In addition, the present disclosure describes a method of hybrid capture that does not involve amplifying the library member using PCR prior to sequencing the library member.