METHOD OF OBTAINING SPROUTS BY WAY OF SOMATIC EMBRYOGENESIS FROM FRAGMENTS OF LEAF BLADES WITH ECHINACEA PURPUREA L. (MOENCH)

摘要

The idea of the invention is obtaining Echinacea purpurea sprouts from fragments of leaf blades by way of somatic embryogenesis in in vitro cultures destined for further greenhouse cultivation, garden growing, production of secondary metabolites or creative breeding of new species in which plant material in the form of sterile fragments of leaf blades is placed on MS medium (1962) modified by increasing by 1/2 of iron chelate and calcium chloride and adding to the medium of growth regulators in the form of BAP cytokinin (6-benzylaminopurine) in the concentration of 0.5 - 1 mg/ L and auxin: IAA (3-indolylacetic acid) in the concentration of 0.5 - 1 mg/ L.