A protein containing the Shiga toxin A subunit effector region and the cell-targeted immunoglobulin-type binding region located proximal to the amino terminus.

摘要

The present invention relates to an immunoglobulin-type binding region for cell-type specific targeting, and Shiga toxin for Shiga toxin effector function (eg, cell internalization, intracellular routing instructions and / or cytotoxicity). An A subunit effector region is provided, and the binding region and Shiga toxin effector region are combined such that the Shiga toxin effector region is proximal to the amino terminus of the protein. The proteins disclosed herein may contain additional exogenous substances, such as antigens, cytotoxic substances, detection enhancers, etc., and these additional exogenous substances are targeted into the target cells. Delivery is possible. The proteins of the invention can be used, for example, for targeted killing of target cells, delivery of exogenous substances to target cells, labeling of intracellular compartments of target cells, and cancer, tumors, other proliferative disorders, immune disorders, and Used in methods such as methods involving diagnosis and / or treatment of various conditions such as microbial infections.