A Method for Designing a Fusion Protein Comprising DNA Binding Protein Containing PPR Motif

摘要

Based on the prediction that RNA recognition rules having a PPR motif can also be used for DNA recognition, the subject is to analyze PPR proteins acting on DNA binding and to search for PPR proteins having such characteristics. The present invention is a PPR motif having the structure of the following general formula (1): (Helix A)-X-(Helix B)-L general formula (1) (wherein, Helix A is a moiety capable of forming an α-helix structure ; X is absent or is a moiety consisting of 1 to 9 amino acids; Helix B is a moiety capable of forming an α-helix structure; And, L is a moiety consisting of 2 to 7 amino acids), and the general A combination of specific amino acids corresponding to the target DNA base or target nucleotide sequence in which three amino acids of amino acid No. 1, amino acid No. 4 of Helix A of formula (1), and amino acid “ii” (-2) included in L are A plurality of PPR motifs having, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, and DNA base selectively or DNA sequence specific binding possible It has been found that proteins can solve this problem.