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PREPARATION OF GLYCOSYLTRANSFERASE UGT76G1 MUTANT AND USE THEREOF
PREPARATION OF GLYCOSYLTRANSFERASE UGT76G1 MUTANT AND USE THEREOF
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机译:糖基转移酶UGT76G1突变体的制备及其用途
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摘要
Provided are a glycosyltransferase UGT76G1 mutant, an isolated polynucleotide, a carrier, a production method, and a composition and a kit for the glycosylation of substrates. Further provided are a method for increasing the catalytic activity of the glycosyltransferase UGT76G1 mutant, and a method for promoting the glycosylation of flavone compounds and the use thereof. The glycosyltransferase UGT76G1 mutant is subjected to mutations at positions corresponding to positions 87, 199, 204 or 379 of SEQ ID NO: 1, and significantly improves the transformation activity of substrates of the flavone compounds.
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机译:提供糖基转移酶UGT76G1突变体,分离的多核苷酸,载体,制备方法和组合物,以及用于基质糖基化的试剂盒。进一步提供的是用于增加糖基转移酶UGT76G1突变体的催化活性的方法,以及用于促进黄酮化合物的糖基化的方法及其用途。将糖基转移酶UGT76G1突变体在对应于SEQ ID NO:1的位置87,199,204或379的位置进行突变,并显着改善黄酮化合物的底物的转化活性。
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