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METHODS TO PROFILE MOLECULAR COMPLEXES BY USING PROXIMITY DEPENDANT BAR-CODING
METHODS TO PROFILE MOLECULAR COMPLEXES BY USING PROXIMITY DEPENDANT BAR-CODING
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机译:通过使用邻近依赖性条形码进行分子复合物的方法
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摘要
Method for studying constituents of individual molecular complexes by labelling the molecules belonging to the same complex with at least one set of molecular constructs, wherein each set member includes a Unique Identifying Sequence (UIS), which is a nucleic acid sequence unique for each set member, and at least one Common Tag Sequence (CTS), which is a nucleic acid sequence common to all set members, by: attaching the molecular construct to the complex by ligating or hybridizing the molecular construct to a nucleic acid molecule of the complex, or ligating or hybridizing the tag to a nucleic acid linked to an affinity binder that binds specifically to a constituent of the complex; labelling the molecules belonging to the same complex using the molecular construct tags as primers or templates in a nucleic acid polymerization reaction; and analyzing the composition of the complex by analyzing combinations of UISs and CTSs.
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