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LC-MS METHODS FOR ANTIBODY ISOTYPING AND QUANTIFICATION
LC-MS METHODS FOR ANTIBODY ISOTYPING AND QUANTIFICATION
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机译:LC-MS用于抗体异型和定量的方法
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摘要
The present invention provides methods and systems for isotyping and quantification of antibodies based on immunocapture and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. These antibodies are induced by the administration of pharmaceutical products. The immunocapture method comprises contacting samples with a solid support, wherein the pharmaceutical product has been cross-linked directly to the solid support. The MS analysis includes conducting peptide mapping, selecting unique peptides and fragment ions to generate MRM (multiple reaction monitoring) transitions, optimizing collision energy, and determining a LLOQ (lower limit of quantification).
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