Metabolically modified organisms for the production of bio-products with added value

摘要

Metabolically modified bacterium or metabolically modified yeast for the production of a specific carbohydrate product, characterized by the fact that the bacterium or yeast:(a) has been genetically modified by the introduction of a heterologous gene coding a sucrose phosphorylase capable of splitting sucrose into glucose-1 phosphate and fructose, or a heterologous gene coding a carbohydrate hydrolase capable of splitting sucrose into glucose and fructose;(b) includes a fructokinase to catalyse the conversion of fructose into fructose 6-phosphate; and(c) has been further genetically modified to prevent the loss of fructose 6-phosphate via glycolysis due to the destruction of an endogenous gene encoding a phosphofructokinase, a phosphoglucose isomerase or a combination thereof.