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COMBINATORIAL ASSEMBLY OF COMPOSITE ARRAYS OF SITE-SPECIFIC SYNTHETIC TRANSPOSONS INSERTED INTO SEQUENCES COMPRISING NOVEL TARGET SITES IN MODULAR PROKARYOTIC AND EUKARYOTIC VECTORS
COMBINATORIAL ASSEMBLY OF COMPOSITE ARRAYS OF SITE-SPECIFIC SYNTHETIC TRANSPOSONS INSERTED INTO SEQUENCES COMPRISING NOVEL TARGET SITES IN MODULAR PROKARYOTIC AND EUKARYOTIC VECTORS
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机译:组合组合阵列的组合组合阵列,其插入序列,该序列包括模块化原核和真核载体中的新靶位点
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摘要
The design, assembly, and use of novel sequences comprising targeting and insertion sites for site-specific bacterial transposons are disclosed. One aspect relates to a nucleotide sequence comprising an attachment site for a site-specific transposon operably-linked to a screenable or selectable marker sequence, wherein said marker sequence encodes one or more active or inactive polypeptides capable of conferring a screenable or selectable phenotype upon a cell comprising the marker sequence, wherein insertion of the site-specific transposon into the attachment site changes the phenotype of a cell comprising the screenable or selectable marker sequence. High and low copy number vectors comprising the sequences, designated synthemids, including plasmids capable of propagating in bacteria, and shuttle vectors, capable of propagating in bacteria and a eukaryotic host cell or two types of bacteria by means of distinct replicons, are also disclosed.
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