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METHODS AND SYSTEMS FOR THE CULTURE OF CELLS AT LIQUID-LIQUID INTERFACES
METHODS AND SYSTEMS FOR THE CULTURE OF CELLS AT LIQUID-LIQUID INTERFACES
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机译:液 - 液界面细胞培养的方法和系统
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摘要
The present invention relates to methods of culturing adherent cells, in particular adherent stem cells, to confluency at a liquid-liquid interface. The invention also provides cell culture systems useful in the culture of cells at liquid-liquid interfaces. The cell culture systems generally comprise an aqueous cell culture medium and an oil phase, there being a conditioning layer disposed between the cell culture medium and the oil phase comprising a peptide or polymer layer and a surfactant that assists in the culture of the adherent cells (in particular stem cells) at the interface between the two phases.
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