METHOD FOR IDENTIFYING POTENTIAL DRUG TARGET PROTEIN USEFUL FOR DEVELOPMENT OF ANTIBODY DRUG, AND METHOD FOR PRODUCING ANTIBODY DIRECTED AGAINST TARGET PROTEIN

摘要

An object of the present invention is, regarding production of an antibody useful in the pharmaceutical and agricultural fields, to find a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. The object of the present invention can be achieved by producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.