To provide a route selection assistance system, a recording medium on which a route selection assistance program is recorded, a route selection assistance method, and a diagnosis method that enable easy selection of a route of a living body lumen for delivering a medical instrument to a site within a living body via the living body lumen. A route selection assistance system 1 includes: a receiving section 110 configured to receive an input of site information specifying a target site; an image obtaining section 120 configured to obtain image information on a living body of a target patient; a route extracting section 130 configured to extract a plurality of routes of a living body lumen, the plurality of routes allowing a medical instrument to be delivered to the target site, on a basis of the image information; a ranking assigning section 140 configured to assign rankings to the plurality of routes extracted by the route extracting section by using route scores determined according to ease of delivery of the medical instrument at a time of delivery of the medical instrument and patient scores determined according to magnitude of a burden imposed on the target patient; and an output section 150 configured to output the plurality of routes extracted by the route extracting section and the rankings assigned by the ranking assigning section, the image information including an imaging image of the living body lumen, and a route score calculating section calculating the route scores by using the imaging image.
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